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MCB2289l Week 11 Final Exam 2018 (A++++ Answer)
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QUESTION 1

The process of forming mRNA from the template strand of DNA is called _____________

Transcription

Replication

Duplication

Translation

QUESTION 2

Which of the following sequences would result from the replication of the following DNA sequence: ATTGCTGGA

ATTGCTGGA

TTACCGCCA

TAACGACCT

TAACGTCCA

QUESTION 3

Which test uses a medium that is both selective and differential for members of the genus Staphylococcus?

MacConkey agar

Mannitol Salt agar

Coagulase test

Bile esculin hydrolysis

QUESTION 4

Which of the following processes removes or destroys endospores?

Antisepsis

Disinfection

Sterilization

Sanitization

QUESTION 5

The purpose of heat fixation is to _______.

Adjust the morphology of the organisms on the slide

Encourage growth of bacterial cells on the slide

Inactivate and adhere microorganisms placed on the slide

Harden the bacteria so that they are not destroyed by the staining procedure

QUESTION 6

Eukaryotes have membrane-bound organelles

True

False

QUESTION 7

A pure culture contains

a known number of organisms

two types of known microorganisms

one type of microorganism

QUESTION 8

The internationally recognized reference for bacterial classification and identification is called Bergey's Manual

True

False

QUESTION 9

How is a glass culture tube handled during aseptic transfers?

The mouth is held in the flame while the inoculating tool is inserted for removal of organisms

The cap is removed and placed on the table away from the flame

The bottom of the tube is held in the flame for 10 seconds

The mouth is passed through the flame right when opened and just before closing

QUESTION 10

How is a respirometer used to measure fermentation in the Cellular Respiration experiment?

By the amount of glucose consumed

By the size of the gas bubble produced

By the amount of ATP produced

By the amount of gas consumed

QUESTION 11

Which organelle is involved in the packaging of proteins for secretion?

Rough endoplasmic reticulum

Nucleus

Golgi apparatus

Mitochondrion

QUESTION 12

Which statement correctly describes the level of contamination on different objects?

Different objects will increase in their level of contamination with described temperature

Different objects will have the same level of contamination.

Environments in contact with animals and humans are likely to be less contaminated due to hygiene practices.

Different objects will vary in their level of contamination.

QUESTION 13

Which type of reaction(s) requires oxygen as a substrate?

Anaerobic

Aerobic

Both aerobic and anaerobic reactions require oxygen

Neither reactions require oxygen

QUESTION 14

Catalase breaks hydrogen peroxide down in water and carbon dioxide

True

False

QUESTION 15

Which of the following is true of a bacterial species that produces dark colonies when grown on MacConkey agar.

They are glucose fermenters

They are lactose fermenters

They produce urease

They reduce nitrate to nitrite

QUESTION 16

Phenol red carbohydrate broths with Durham tubes can detect all of the following EXCEPT

Nitrate reduction

Acid production

Gas production

Fermentation

QUESTION 17

All of the following are common to all cells at some point in their development EXCEPT:

DNA

The cell wall

The cell membrane

Ribosomes

QUESTION 18

In the Gram stain, the chemical that fixes dye in or on cells by forming an insoluble compound in referred to as the _______________?

mordant

decolorizer

counterstain

primary stain

QUESTION 19

All of the following are true of an enzyme-catalyzed reaction EXCEPT?

An enzyme reduces the activation energy

Every time a substrate is converted to a product, a bit of the enzyme is used up

The enzyme speeds up the conversion of substrate to product

Enzymes are not used up in the chemical reaction

QUESTION 20

Which of the following diseases are caused by acid-fast bacterium:

HIV

Influenza

Cholera

Tuberculosis (TB)

QUESTION 21

If an organism produces stable acids as an end-product of glucose fermentation, the methyl red test will be red, indicating a positive result.

True

False

QUESTION 22

Immersion oil must only be used with which objective lens?

4x

10x

40x

100x

QUESTION 23

An antimicrobial, such as isoniazid, that is only effective against mycobacterial species would be referred to as a __________ drug.

Broad spectrum          

Narrow spectrum

Intermediate spectrum

Naturally occurring

QUESTION 24

Most human pathogens are classified as thermophiles.

True

False

QUESTION 25

In living organisms, information for making proteins flows from _______?

RNA to DNA to protein.

RNA to protein to DNA

DNA to RNA to protein

Protein to RNA to DNA

QUESTION 26

Microorganisms can be described as being ubiquitous which means that they:

All grow uniformly on an agar plate

Are present on nearly every surface

Can only grow on solid surfaces

Can only grow in the absence of oxygen

QUESTION 27

Stains are commonly used for all of the following EXCEPT

The pathogen identification process

The diagnosis of disease

The classification of organisms

The treatment of disease

QUESTION 28

The monomers that make up DNA molecules are called _______?

Sugars

Amino acids

Nucleotides

Nitrogenous bases

QUESTION 29

Which of the following is a chemical means of controlling microbial growth?

Disinfectant

High temperature

Scrubbing action

Ultraviolet radiation

QUESTION 30

All of the following organisms are expected to grow within an anaerobe jar EXCEPT

Obligate anaerobe

Aerotolerant anaerobe

Obligate aerobe

Facultative anaerobe

QUESTION 31

The cloudy growth seen in a broth culture is referred to as

Precipitate

Pellicle

Sediment

Turbidity

QUESTION 32

An antibiotic which interferes with the activity of ribosomes would have the most effect on which of the following?

DNA replication

The cell wall

Protein synthesis

Cell membrane function

QUESTION 33

When a large zone of no growth is found around an antibiotic disc being used for identification purposed, this generally means that the microbe is ______ to the antibiotic

Resistant

Pathogenic

Sensitive

Neutral

QUESTION 34

All of the following are methods that can be used to isolate bacteria from a mixed culture EXCEPT?

Streak plating

Loop dilution pour plating

Gram staining

Serial dilution followed by spread plating

QUESTION 35

The smallest living units of the human body are __________.

molecules

atoms

tissues

cells

QUESTION 36

When first viewing a specimen using the brightfield microscope, it is recommended that you begin viewing the specimen with which objective lens?

100X

40X

10X

4X (scanning objective)

QUESTION 37

Before attempting to identify an unknown bacterial species, the organism must be isolated in a pure culture.

True

False

QUESTION 38

In addition to linking together nucleotides to synthesize a daughter strand, DNA polymerase also ________

Unzips the parent DNA molecule

"proofreads" the order of nucleotides

Makes the new DNA strand form a helix

"zips" the parent molecule back together

QUESTION 39

All of the following are found in most prokaryotic cells EXCEPT

Cell membrane

Cytoplasm

Cell wall

Nucleus

QUESTION 40

The sources of all antimicrobial drugs are fungi and other microorganisms.

True

False

QUESTION 41

Which carbohydrate is the primary or preferred starting compound for glycolysis and the fermentation pathway?

Sucrose

Glucose

Lactose

fructose

QUESTION 42

All of the following body sites normally have large numbers of resident microbiota EXCEPT?

Nose

Large intestine

Skin

Blood

QUESTION 43

Human skin provides an environment with NaCl levels slightly above that of most bacterial cells. Which organism would most likely be found on the skin?

A halotolerant organism

An organism that does not tolerate hypertonic salt conditions very well

An obligate anaerobe

A psychrophile

QUESTION 44

Gram negative cells appear purple after Gram staining whereas gram positive cells appear pink

True

False

QUESTION 45

An organism has been shown to produce hydrogen sulfide and indole in its metabolism. These are examples of _____ characteristics that can be helpful in the identification of the microorganism.

Morphological

Genotypic

Physiological

Immunological

QUESTION 46

A hypertonic solution is prepared by the addition of sucrose. Which of the following would happen to bacteria placed in this solution:

The bacterial cells will swell, but not lyse

There will be no change in the bacterial cells

The bacterial cells will undergo osmotic lysis

The bacterial cells will undergo plasmolysis

QUESTION 47

Which of the following items would be disposed of into the sharps container?

Used paper towels

Used gloves

Bacterial culture plates

Used microscope slides

QUESTION 48

When is it crucial to wear protective eyewear when working in the lab?

When using the microscope

When using a Bunsen burner

When working with hazardous chemicals

When working with sharp objects

QUESTION 49

The term aerobic, anaerobic, and microaerophilic are used to categorize microorganisms based on their ______ requirements.

oxygen

temperature

carbon dioxide

pH

QUESTION 50

The ability to distinguish fine detail or discriminate between two very closely spaced objects is called:

Microscopy

Resolution

Clarification

magnification

 

 

 

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MCB2289l Week 11 Final Exam 2018 (A++++ Answer)
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Question QUESTION 1 The process of forming mRNA from the template strand of DNA is called _____________ Transcription Replication Duplication Translation QUESTION 2 Which of the following sequences would result from the replication of the following DNA sequence: ATTGCTGGA ATTGCTGGA TTACCGCCA TAACGACCT TAACGTCCA QUESTION 3 Which test uses a medium that is both selective and differential for members of the genus Staphylococcus? MacConkey agar Mannitol Salt agar Coagulase test Bile esculin hydrolysis QUESTION 4 Which of the following processes removes or destroys endospores? Antisepsis Disinfection Sterilization Sanitization QUESTION 5 The purpose of heat fixation is to _______. Adjust the morphology of the organisms on the slide Encourage growth of bacterial cells on the slide Inactivate and adhere microorganisms placed on the slide Harden the bacteria so that they are not destroyed by the staining procedure QUESTION 6 Eukaryotes have membrane-bound organelles True False QUESTION 7 A pure culture contains a known number of organisms two types of known microorganisms one type of microorganism QUESTION 8 The internationally recognized reference for bacterial classification and identification is called Bergey's Manual True False QUESTION 9 How is a glass culture tube handled during aseptic transfers? The mouth is held in the flame while the inoculating tool is inserted for removal of organisms The cap is removed and placed on the table away from the flame The bottom of the tube is held in the flame for 10 seconds The mouth is passed through the flame right when opened and just before closing QUESTION 10 How is a respirometer used to measure fermentation in the Cellular Respiration experiment? By the amount of glucose consumed By the size of the gas bubble produced By the amount of ATP produced By the amount of gas consumed QUESTION 11 Which organelle is involved in the packaging of proteins for secretion? Rough endoplasmic reticulum Nucleus Golgi apparatus Mitochondrion QUESTION 12 Which statement correctly describes the level of contamination on different objects? Different objects will increase in their level of contamination with described temperature Different objects will have the same level of contamination. Environments in contact w...
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